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feng zhang n a pspax2 laboratory  (Addgene inc)


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    Addgene inc feng zhang n a pspax2 laboratory
    Feng Zhang N A Pspax2 Laboratory, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 15719 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/feng+zhang+n+a+pspax2+laboratory/psPAX2+(Plasmid+%2312260)/pm29551272-226-250-258
    Average 98 stars, based on 15719 article reviews
    feng zhang n a pspax2 laboratory - by Bioz Stars, 2026-11
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    cDNA Synthesis:

    Article Title: Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polyethylenimine Max (PEI) Polysciences Cat# 24765 UltraCULTURE Serum-free Medium Lonza Cat# 12-725F Critical Commercial Assays NEBNext Ultra II Directional RNA Library Prep Kit NEB Cat# E7760 TaqMan Fast Advanced Master Mix Thermo Fisher Cat# 4444556 RNeasy Plus Mini Kit QIAGEN Cat# 74134 RevertAid First Strand cDNA Synthesis Kit Thermo Fisher Cat# K1622 MEGAclear Transcription Clean-Up Kit Thermo Fisher Cat# AM1908 Directzol Miniprep Kit Zymo Research Cat# R2050 Ribo-Zero rRNA Removal Kit (Bacteria) Illumina Cat# MRZMB126 HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040 PEG Virus Precipitation Kit Biovision Cat# K904 Deposited Data GEO (RNA Sequencing of targeting by CasRx and matched shRNAs in 293FT cells) This paper GSE108519 Experimental Models: Cell Lines Human: HEK293FT (Female) Thermo Fisher Cat # R70007 Human: FTD patient iPSC line 3L6 (Male) Laboratory of Fen-Biao Gao Biswas et al., 2016 Human: Control iPSC line 2L17 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Human: Control iPSC line 2L20 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Oligonucleotides Guide and target RNA sequences for biochemical assays, see: Table S1 This paper N/A Guide RNAs for mammalian RNA targeting, see: Table S2 This paper N/A shRNA sequences, see: Table S3 This paper N/A qPCR probes and primers, see: Table S4 This paper N/A Recombinant DNA CRISPR Effector sequences, see: Table S5 This paper N/A pAAV1 Penn Vector Core N/A pAd DeltaF6 Penn Vector Core N/A RG6-mTagBFP2 This paper Adapted from Addgene #80167 from Thomas Cooper His-MBP-TEV Laboratory of Feng Zhang N/A psPAX2 Laboratory of Didier Trono Addgene #12260 pMD2.G Laboratory of Didier Trono Addgene #12259 EF1a-PspCas13b-NES(HIV)-2A-EGFP This paper Adapted from Addgene #103862 from Feng Zhang pC0043-PspCas13b crRNA backbone Cox et al., 2017 Addgene #103854 pC016-LwCas13a guide expression backbone with U6 promoter Abudayyeh et al., 2017 Addgene #91906 EF1a-LwaCas13a-msfGFP-NLS This paper Adapted from Addgene #91924 from Feng Zhang Software and Algorithms Bowtie 2 Langmead and Salzberg, 2012 http://bowtie-bio.sourceforge.net/bowtie2 CRISPRfinder Grissa et al., 2007 Source code was a gift from Christine Pourcel.

    Bacteria:

    Article Title: Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polyethylenimine Max (PEI) Polysciences Cat# 24765 UltraCULTURE Serum-free Medium Lonza Cat# 12-725F Critical Commercial Assays NEBNext Ultra II Directional RNA Library Prep Kit NEB Cat# E7760 TaqMan Fast Advanced Master Mix Thermo Fisher Cat# 4444556 RNeasy Plus Mini Kit QIAGEN Cat# 74134 RevertAid First Strand cDNA Synthesis Kit Thermo Fisher Cat# K1622 MEGAclear Transcription Clean-Up Kit Thermo Fisher Cat# AM1908 Directzol Miniprep Kit Zymo Research Cat# R2050 Ribo-Zero rRNA Removal Kit (Bacteria) Illumina Cat# MRZMB126 HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040 PEG Virus Precipitation Kit Biovision Cat# K904 Deposited Data GEO (RNA Sequencing of targeting by CasRx and matched shRNAs in 293FT cells) This paper GSE108519 Experimental Models: Cell Lines Human: HEK293FT (Female) Thermo Fisher Cat # R70007 Human: FTD patient iPSC line 3L6 (Male) Laboratory of Fen-Biao Gao Biswas et al., 2016 Human: Control iPSC line 2L17 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Human: Control iPSC line 2L20 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Oligonucleotides Guide and target RNA sequences for biochemical assays, see: Table S1 This paper N/A Guide RNAs for mammalian RNA targeting, see: Table S2 This paper N/A shRNA sequences, see: Table S3 This paper N/A qPCR probes and primers, see: Table S4 This paper N/A Recombinant DNA CRISPR Effector sequences, see: Table S5 This paper N/A pAAV1 Penn Vector Core N/A pAd DeltaF6 Penn Vector Core N/A RG6-mTagBFP2 This paper Adapted from Addgene #80167 from Thomas Cooper His-MBP-TEV Laboratory of Feng Zhang N/A psPAX2 Laboratory of Didier Trono Addgene #12260 pMD2.G Laboratory of Didier Trono Addgene #12259 EF1a-PspCas13b-NES(HIV)-2A-EGFP This paper Adapted from Addgene #103862 from Feng Zhang pC0043-PspCas13b crRNA backbone Cox et al., 2017 Addgene #103854 pC016-LwCas13a guide expression backbone with U6 promoter Abudayyeh et al., 2017 Addgene #91906 EF1a-LwaCas13a-msfGFP-NLS This paper Adapted from Addgene #91924 from Feng Zhang Software and Algorithms Bowtie 2 Langmead and Salzberg, 2012 http://bowtie-bio.sourceforge.net/bowtie2 CRISPRfinder Grissa et al., 2007 Source code was a gift from Christine Pourcel.

    Virus:

    Article Title: Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polyethylenimine Max (PEI) Polysciences Cat# 24765 UltraCULTURE Serum-free Medium Lonza Cat# 12-725F Critical Commercial Assays NEBNext Ultra II Directional RNA Library Prep Kit NEB Cat# E7760 TaqMan Fast Advanced Master Mix Thermo Fisher Cat# 4444556 RNeasy Plus Mini Kit QIAGEN Cat# 74134 RevertAid First Strand cDNA Synthesis Kit Thermo Fisher Cat# K1622 MEGAclear Transcription Clean-Up Kit Thermo Fisher Cat# AM1908 Directzol Miniprep Kit Zymo Research Cat# R2050 Ribo-Zero rRNA Removal Kit (Bacteria) Illumina Cat# MRZMB126 HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040 PEG Virus Precipitation Kit Biovision Cat# K904 Deposited Data GEO (RNA Sequencing of targeting by CasRx and matched shRNAs in 293FT cells) This paper GSE108519 Experimental Models: Cell Lines Human: HEK293FT (Female) Thermo Fisher Cat # R70007 Human: FTD patient iPSC line 3L6 (Male) Laboratory of Fen-Biao Gao Biswas et al., 2016 Human: Control iPSC line 2L17 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Human: Control iPSC line 2L20 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Oligonucleotides Guide and target RNA sequences for biochemical assays, see: Table S1 This paper N/A Guide RNAs for mammalian RNA targeting, see: Table S2 This paper N/A shRNA sequences, see: Table S3 This paper N/A qPCR probes and primers, see: Table S4 This paper N/A Recombinant DNA CRISPR Effector sequences, see: Table S5 This paper N/A pAAV1 Penn Vector Core N/A pAd DeltaF6 Penn Vector Core N/A RG6-mTagBFP2 This paper Adapted from Addgene #80167 from Thomas Cooper His-MBP-TEV Laboratory of Feng Zhang N/A psPAX2 Laboratory of Didier Trono Addgene #12260 pMD2.G Laboratory of Didier Trono Addgene #12259 EF1a-PspCas13b-NES(HIV)-2A-EGFP This paper Adapted from Addgene #103862 from Feng Zhang pC0043-PspCas13b crRNA backbone Cox et al., 2017 Addgene #103854 pC016-LwCas13a guide expression backbone with U6 promoter Abudayyeh et al., 2017 Addgene #91906 EF1a-LwaCas13a-msfGFP-NLS This paper Adapted from Addgene #91924 from Feng Zhang Software and Algorithms Bowtie 2 Langmead and Salzberg, 2012 http://bowtie-bio.sourceforge.net/bowtie2 CRISPRfinder Grissa et al., 2007 Source code was a gift from Christine Pourcel.

    RNA Sequencing:

    Article Title: Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polyethylenimine Max (PEI) Polysciences Cat# 24765 UltraCULTURE Serum-free Medium Lonza Cat# 12-725F Critical Commercial Assays NEBNext Ultra II Directional RNA Library Prep Kit NEB Cat# E7760 TaqMan Fast Advanced Master Mix Thermo Fisher Cat# 4444556 RNeasy Plus Mini Kit QIAGEN Cat# 74134 RevertAid First Strand cDNA Synthesis Kit Thermo Fisher Cat# K1622 MEGAclear Transcription Clean-Up Kit Thermo Fisher Cat# AM1908 Directzol Miniprep Kit Zymo Research Cat# R2050 Ribo-Zero rRNA Removal Kit (Bacteria) Illumina Cat# MRZMB126 HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040 PEG Virus Precipitation Kit Biovision Cat# K904 Deposited Data GEO (RNA Sequencing of targeting by CasRx and matched shRNAs in 293FT cells) This paper GSE108519 Experimental Models: Cell Lines Human: HEK293FT (Female) Thermo Fisher Cat # R70007 Human: FTD patient iPSC line 3L6 (Male) Laboratory of Fen-Biao Gao Biswas et al., 2016 Human: Control iPSC line 2L17 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Human: Control iPSC line 2L20 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Oligonucleotides Guide and target RNA sequences for biochemical assays, see: Table S1 This paper N/A Guide RNAs for mammalian RNA targeting, see: Table S2 This paper N/A shRNA sequences, see: Table S3 This paper N/A qPCR probes and primers, see: Table S4 This paper N/A Recombinant DNA CRISPR Effector sequences, see: Table S5 This paper N/A pAAV1 Penn Vector Core N/A pAd DeltaF6 Penn Vector Core N/A RG6-mTagBFP2 This paper Adapted from Addgene #80167 from Thomas Cooper His-MBP-TEV Laboratory of Feng Zhang N/A psPAX2 Laboratory of Didier Trono Addgene #12260 pMD2.G Laboratory of Didier Trono Addgene #12259 EF1a-PspCas13b-NES(HIV)-2A-EGFP This paper Adapted from Addgene #103862 from Feng Zhang pC0043-PspCas13b crRNA backbone Cox et al., 2017 Addgene #103854 pC016-LwCas13a guide expression backbone with U6 promoter Abudayyeh et al., 2017 Addgene #91906 EF1a-LwaCas13a-msfGFP-NLS This paper Adapted from Addgene #91924 from Feng Zhang Software and Algorithms Bowtie 2 Langmead and Salzberg, 2012 http://bowtie-bio.sourceforge.net/bowtie2 CRISPRfinder Grissa et al., 2007 Source code was a gift from Christine Pourcel.

    Control:

    Article Title: Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polyethylenimine Max (PEI) Polysciences Cat# 24765 UltraCULTURE Serum-free Medium Lonza Cat# 12-725F Critical Commercial Assays NEBNext Ultra II Directional RNA Library Prep Kit NEB Cat# E7760 TaqMan Fast Advanced Master Mix Thermo Fisher Cat# 4444556 RNeasy Plus Mini Kit QIAGEN Cat# 74134 RevertAid First Strand cDNA Synthesis Kit Thermo Fisher Cat# K1622 MEGAclear Transcription Clean-Up Kit Thermo Fisher Cat# AM1908 Directzol Miniprep Kit Zymo Research Cat# R2050 Ribo-Zero rRNA Removal Kit (Bacteria) Illumina Cat# MRZMB126 HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040 PEG Virus Precipitation Kit Biovision Cat# K904 Deposited Data GEO (RNA Sequencing of targeting by CasRx and matched shRNAs in 293FT cells) This paper GSE108519 Experimental Models: Cell Lines Human: HEK293FT (Female) Thermo Fisher Cat # R70007 Human: FTD patient iPSC line 3L6 (Male) Laboratory of Fen-Biao Gao Biswas et al., 2016 Human: Control iPSC line 2L17 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Human: Control iPSC line 2L20 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Oligonucleotides Guide and target RNA sequences for biochemical assays, see: Table S1 This paper N/A Guide RNAs for mammalian RNA targeting, see: Table S2 This paper N/A shRNA sequences, see: Table S3 This paper N/A qPCR probes and primers, see: Table S4 This paper N/A Recombinant DNA CRISPR Effector sequences, see: Table S5 This paper N/A pAAV1 Penn Vector Core N/A pAd DeltaF6 Penn Vector Core N/A RG6-mTagBFP2 This paper Adapted from Addgene #80167 from Thomas Cooper His-MBP-TEV Laboratory of Feng Zhang N/A psPAX2 Laboratory of Didier Trono Addgene #12260 pMD2.G Laboratory of Didier Trono Addgene #12259 EF1a-PspCas13b-NES(HIV)-2A-EGFP This paper Adapted from Addgene #103862 from Feng Zhang pC0043-PspCas13b crRNA backbone Cox et al., 2017 Addgene #103854 pC016-LwCas13a guide expression backbone with U6 promoter Abudayyeh et al., 2017 Addgene #91906 EF1a-LwaCas13a-msfGFP-NLS This paper Adapted from Addgene #91924 from Feng Zhang Software and Algorithms Bowtie 2 Langmead and Salzberg, 2012 http://bowtie-bio.sourceforge.net/bowtie2 CRISPRfinder Grissa et al., 2007 Source code was a gift from Christine Pourcel.

    shRNA:

    Article Title: Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polyethylenimine Max (PEI) Polysciences Cat# 24765 UltraCULTURE Serum-free Medium Lonza Cat# 12-725F Critical Commercial Assays NEBNext Ultra II Directional RNA Library Prep Kit NEB Cat# E7760 TaqMan Fast Advanced Master Mix Thermo Fisher Cat# 4444556 RNeasy Plus Mini Kit QIAGEN Cat# 74134 RevertAid First Strand cDNA Synthesis Kit Thermo Fisher Cat# K1622 MEGAclear Transcription Clean-Up Kit Thermo Fisher Cat# AM1908 Directzol Miniprep Kit Zymo Research Cat# R2050 Ribo-Zero rRNA Removal Kit (Bacteria) Illumina Cat# MRZMB126 HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040 PEG Virus Precipitation Kit Biovision Cat# K904 Deposited Data GEO (RNA Sequencing of targeting by CasRx and matched shRNAs in 293FT cells) This paper GSE108519 Experimental Models: Cell Lines Human: HEK293FT (Female) Thermo Fisher Cat # R70007 Human: FTD patient iPSC line 3L6 (Male) Laboratory of Fen-Biao Gao Biswas et al., 2016 Human: Control iPSC line 2L17 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Human: Control iPSC line 2L20 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Oligonucleotides Guide and target RNA sequences for biochemical assays, see: Table S1 This paper N/A Guide RNAs for mammalian RNA targeting, see: Table S2 This paper N/A shRNA sequences, see: Table S3 This paper N/A qPCR probes and primers, see: Table S4 This paper N/A Recombinant DNA CRISPR Effector sequences, see: Table S5 This paper N/A pAAV1 Penn Vector Core N/A pAd DeltaF6 Penn Vector Core N/A RG6-mTagBFP2 This paper Adapted from Addgene #80167 from Thomas Cooper His-MBP-TEV Laboratory of Feng Zhang N/A psPAX2 Laboratory of Didier Trono Addgene #12260 pMD2.G Laboratory of Didier Trono Addgene #12259 EF1a-PspCas13b-NES(HIV)-2A-EGFP This paper Adapted from Addgene #103862 from Feng Zhang pC0043-PspCas13b crRNA backbone Cox et al., 2017 Addgene #103854 pC016-LwCas13a guide expression backbone with U6 promoter Abudayyeh et al., 2017 Addgene #91906 EF1a-LwaCas13a-msfGFP-NLS This paper Adapted from Addgene #91924 from Feng Zhang Software and Algorithms Bowtie 2 Langmead and Salzberg, 2012 http://bowtie-bio.sourceforge.net/bowtie2 CRISPRfinder Grissa et al., 2007 Source code was a gift from Christine Pourcel.

    Real-time Polymerase Chain Reaction:

    Article Title: Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polyethylenimine Max (PEI) Polysciences Cat# 24765 UltraCULTURE Serum-free Medium Lonza Cat# 12-725F Critical Commercial Assays NEBNext Ultra II Directional RNA Library Prep Kit NEB Cat# E7760 TaqMan Fast Advanced Master Mix Thermo Fisher Cat# 4444556 RNeasy Plus Mini Kit QIAGEN Cat# 74134 RevertAid First Strand cDNA Synthesis Kit Thermo Fisher Cat# K1622 MEGAclear Transcription Clean-Up Kit Thermo Fisher Cat# AM1908 Directzol Miniprep Kit Zymo Research Cat# R2050 Ribo-Zero rRNA Removal Kit (Bacteria) Illumina Cat# MRZMB126 HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040 PEG Virus Precipitation Kit Biovision Cat# K904 Deposited Data GEO (RNA Sequencing of targeting by CasRx and matched shRNAs in 293FT cells) This paper GSE108519 Experimental Models: Cell Lines Human: HEK293FT (Female) Thermo Fisher Cat # R70007 Human: FTD patient iPSC line 3L6 (Male) Laboratory of Fen-Biao Gao Biswas et al., 2016 Human: Control iPSC line 2L17 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Human: Control iPSC line 2L20 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Oligonucleotides Guide and target RNA sequences for biochemical assays, see: Table S1 This paper N/A Guide RNAs for mammalian RNA targeting, see: Table S2 This paper N/A shRNA sequences, see: Table S3 This paper N/A qPCR probes and primers, see: Table S4 This paper N/A Recombinant DNA CRISPR Effector sequences, see: Table S5 This paper N/A pAAV1 Penn Vector Core N/A pAd DeltaF6 Penn Vector Core N/A RG6-mTagBFP2 This paper Adapted from Addgene #80167 from Thomas Cooper His-MBP-TEV Laboratory of Feng Zhang N/A psPAX2 Laboratory of Didier Trono Addgene #12260 pMD2.G Laboratory of Didier Trono Addgene #12259 EF1a-PspCas13b-NES(HIV)-2A-EGFP This paper Adapted from Addgene #103862 from Feng Zhang pC0043-PspCas13b crRNA backbone Cox et al., 2017 Addgene #103854 pC016-LwCas13a guide expression backbone with U6 promoter Abudayyeh et al., 2017 Addgene #91906 EF1a-LwaCas13a-msfGFP-NLS This paper Adapted from Addgene #91924 from Feng Zhang Software and Algorithms Bowtie 2 Langmead and Salzberg, 2012 http://bowtie-bio.sourceforge.net/bowtie2 CRISPRfinder Grissa et al., 2007 Source code was a gift from Christine Pourcel.

    Recombinant:

    Article Title: Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polyethylenimine Max (PEI) Polysciences Cat# 24765 UltraCULTURE Serum-free Medium Lonza Cat# 12-725F Critical Commercial Assays NEBNext Ultra II Directional RNA Library Prep Kit NEB Cat# E7760 TaqMan Fast Advanced Master Mix Thermo Fisher Cat# 4444556 RNeasy Plus Mini Kit QIAGEN Cat# 74134 RevertAid First Strand cDNA Synthesis Kit Thermo Fisher Cat# K1622 MEGAclear Transcription Clean-Up Kit Thermo Fisher Cat# AM1908 Directzol Miniprep Kit Zymo Research Cat# R2050 Ribo-Zero rRNA Removal Kit (Bacteria) Illumina Cat# MRZMB126 HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040 PEG Virus Precipitation Kit Biovision Cat# K904 Deposited Data GEO (RNA Sequencing of targeting by CasRx and matched shRNAs in 293FT cells) This paper GSE108519 Experimental Models: Cell Lines Human: HEK293FT (Female) Thermo Fisher Cat # R70007 Human: FTD patient iPSC line 3L6 (Male) Laboratory of Fen-Biao Gao Biswas et al., 2016 Human: Control iPSC line 2L17 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Human: Control iPSC line 2L20 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Oligonucleotides Guide and target RNA sequences for biochemical assays, see: Table S1 This paper N/A Guide RNAs for mammalian RNA targeting, see: Table S2 This paper N/A shRNA sequences, see: Table S3 This paper N/A qPCR probes and primers, see: Table S4 This paper N/A Recombinant DNA CRISPR Effector sequences, see: Table S5 This paper N/A pAAV1 Penn Vector Core N/A pAd DeltaF6 Penn Vector Core N/A RG6-mTagBFP2 This paper Adapted from Addgene #80167 from Thomas Cooper His-MBP-TEV Laboratory of Feng Zhang N/A psPAX2 Laboratory of Didier Trono Addgene #12260 pMD2.G Laboratory of Didier Trono Addgene #12259 EF1a-PspCas13b-NES(HIV)-2A-EGFP This paper Adapted from Addgene #103862 from Feng Zhang pC0043-PspCas13b crRNA backbone Cox et al., 2017 Addgene #103854 pC016-LwCas13a guide expression backbone with U6 promoter Abudayyeh et al., 2017 Addgene #91906 EF1a-LwaCas13a-msfGFP-NLS This paper Adapted from Addgene #91924 from Feng Zhang Software and Algorithms Bowtie 2 Langmead and Salzberg, 2012 http://bowtie-bio.sourceforge.net/bowtie2 CRISPRfinder Grissa et al., 2007 Source code was a gift from Christine Pourcel.

    CRISPR:

    Article Title: Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polyethylenimine Max (PEI) Polysciences Cat# 24765 UltraCULTURE Serum-free Medium Lonza Cat# 12-725F Critical Commercial Assays NEBNext Ultra II Directional RNA Library Prep Kit NEB Cat# E7760 TaqMan Fast Advanced Master Mix Thermo Fisher Cat# 4444556 RNeasy Plus Mini Kit QIAGEN Cat# 74134 RevertAid First Strand cDNA Synthesis Kit Thermo Fisher Cat# K1622 MEGAclear Transcription Clean-Up Kit Thermo Fisher Cat# AM1908 Directzol Miniprep Kit Zymo Research Cat# R2050 Ribo-Zero rRNA Removal Kit (Bacteria) Illumina Cat# MRZMB126 HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040 PEG Virus Precipitation Kit Biovision Cat# K904 Deposited Data GEO (RNA Sequencing of targeting by CasRx and matched shRNAs in 293FT cells) This paper GSE108519 Experimental Models: Cell Lines Human: HEK293FT (Female) Thermo Fisher Cat # R70007 Human: FTD patient iPSC line 3L6 (Male) Laboratory of Fen-Biao Gao Biswas et al., 2016 Human: Control iPSC line 2L17 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Human: Control iPSC line 2L20 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Oligonucleotides Guide and target RNA sequences for biochemical assays, see: Table S1 This paper N/A Guide RNAs for mammalian RNA targeting, see: Table S2 This paper N/A shRNA sequences, see: Table S3 This paper N/A qPCR probes and primers, see: Table S4 This paper N/A Recombinant DNA CRISPR Effector sequences, see: Table S5 This paper N/A pAAV1 Penn Vector Core N/A pAd DeltaF6 Penn Vector Core N/A RG6-mTagBFP2 This paper Adapted from Addgene #80167 from Thomas Cooper His-MBP-TEV Laboratory of Feng Zhang N/A psPAX2 Laboratory of Didier Trono Addgene #12260 pMD2.G Laboratory of Didier Trono Addgene #12259 EF1a-PspCas13b-NES(HIV)-2A-EGFP This paper Adapted from Addgene #103862 from Feng Zhang pC0043-PspCas13b crRNA backbone Cox et al., 2017 Addgene #103854 pC016-LwCas13a guide expression backbone with U6 promoter Abudayyeh et al., 2017 Addgene #91906 EF1a-LwaCas13a-msfGFP-NLS This paper Adapted from Addgene #91924 from Feng Zhang Software and Algorithms Bowtie 2 Langmead and Salzberg, 2012 http://bowtie-bio.sourceforge.net/bowtie2 CRISPRfinder Grissa et al., 2007 Source code was a gift from Christine Pourcel.

    Expressing:

    Article Title: Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polyethylenimine Max (PEI) Polysciences Cat# 24765 UltraCULTURE Serum-free Medium Lonza Cat# 12-725F Critical Commercial Assays NEBNext Ultra II Directional RNA Library Prep Kit NEB Cat# E7760 TaqMan Fast Advanced Master Mix Thermo Fisher Cat# 4444556 RNeasy Plus Mini Kit QIAGEN Cat# 74134 RevertAid First Strand cDNA Synthesis Kit Thermo Fisher Cat# K1622 MEGAclear Transcription Clean-Up Kit Thermo Fisher Cat# AM1908 Directzol Miniprep Kit Zymo Research Cat# R2050 Ribo-Zero rRNA Removal Kit (Bacteria) Illumina Cat# MRZMB126 HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040 PEG Virus Precipitation Kit Biovision Cat# K904 Deposited Data GEO (RNA Sequencing of targeting by CasRx and matched shRNAs in 293FT cells) This paper GSE108519 Experimental Models: Cell Lines Human: HEK293FT (Female) Thermo Fisher Cat # R70007 Human: FTD patient iPSC line 3L6 (Male) Laboratory of Fen-Biao Gao Biswas et al., 2016 Human: Control iPSC line 2L17 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Human: Control iPSC line 2L20 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Oligonucleotides Guide and target RNA sequences for biochemical assays, see: Table S1 This paper N/A Guide RNAs for mammalian RNA targeting, see: Table S2 This paper N/A shRNA sequences, see: Table S3 This paper N/A qPCR probes and primers, see: Table S4 This paper N/A Recombinant DNA CRISPR Effector sequences, see: Table S5 This paper N/A pAAV1 Penn Vector Core N/A pAd DeltaF6 Penn Vector Core N/A RG6-mTagBFP2 This paper Adapted from Addgene #80167 from Thomas Cooper His-MBP-TEV Laboratory of Feng Zhang N/A psPAX2 Laboratory of Didier Trono Addgene #12260 pMD2.G Laboratory of Didier Trono Addgene #12259 EF1a-PspCas13b-NES(HIV)-2A-EGFP This paper Adapted from Addgene #103862 from Feng Zhang pC0043-PspCas13b crRNA backbone Cox et al., 2017 Addgene #103854 pC016-LwCas13a guide expression backbone with U6 promoter Abudayyeh et al., 2017 Addgene #91906 EF1a-LwaCas13a-msfGFP-NLS This paper Adapted from Addgene #91924 from Feng Zhang Software and Algorithms Bowtie 2 Langmead and Salzberg, 2012 http://bowtie-bio.sourceforge.net/bowtie2 CRISPRfinder Grissa et al., 2007 Source code was a gift from Christine Pourcel.

    Software:

    Article Title: Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polyethylenimine Max (PEI) Polysciences Cat# 24765 UltraCULTURE Serum-free Medium Lonza Cat# 12-725F Critical Commercial Assays NEBNext Ultra II Directional RNA Library Prep Kit NEB Cat# E7760 TaqMan Fast Advanced Master Mix Thermo Fisher Cat# 4444556 RNeasy Plus Mini Kit QIAGEN Cat# 74134 RevertAid First Strand cDNA Synthesis Kit Thermo Fisher Cat# K1622 MEGAclear Transcription Clean-Up Kit Thermo Fisher Cat# AM1908 Directzol Miniprep Kit Zymo Research Cat# R2050 Ribo-Zero rRNA Removal Kit (Bacteria) Illumina Cat# MRZMB126 HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040 PEG Virus Precipitation Kit Biovision Cat# K904 Deposited Data GEO (RNA Sequencing of targeting by CasRx and matched shRNAs in 293FT cells) This paper GSE108519 Experimental Models: Cell Lines Human: HEK293FT (Female) Thermo Fisher Cat # R70007 Human: FTD patient iPSC line 3L6 (Male) Laboratory of Fen-Biao Gao Biswas et al., 2016 Human: Control iPSC line 2L17 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Human: Control iPSC line 2L20 (Male) Laboratory of Fen-Biao Gao Almeida et al., 2012 Oligonucleotides Guide and target RNA sequences for biochemical assays, see: Table S1 This paper N/A Guide RNAs for mammalian RNA targeting, see: Table S2 This paper N/A shRNA sequences, see: Table S3 This paper N/A qPCR probes and primers, see: Table S4 This paper N/A Recombinant DNA CRISPR Effector sequences, see: Table S5 This paper N/A pAAV1 Penn Vector Core N/A pAd DeltaF6 Penn Vector Core N/A RG6-mTagBFP2 This paper Adapted from Addgene #80167 from Thomas Cooper His-MBP-TEV Laboratory of Feng Zhang N/A psPAX2 Laboratory of Didier Trono Addgene #12260 pMD2.G Laboratory of Didier Trono Addgene #12259 EF1a-PspCas13b-NES(HIV)-2A-EGFP This paper Adapted from Addgene #103862 from Feng Zhang pC0043-PspCas13b crRNA backbone Cox et al., 2017 Addgene #103854 pC016-LwCas13a guide expression backbone with U6 promoter Abudayyeh et al., 2017 Addgene #91906 EF1a-LwaCas13a-msfGFP-NLS This paper Adapted from Addgene #91924 from Feng Zhang Software and Algorithms Bowtie 2 Langmead and Salzberg, 2012 http://bowtie-bio.sourceforge.net/bowtie2 CRISPRfinder Grissa et al., 2007 Source code was a gift from Christine Pourcel.



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    Addgene inc feng zhang n a pspax2 laboratory
    Feng Zhang N A Pspax2 Laboratory, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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